H2N2V1, Main, Exploration, bibRecord, 002A39

Internal proteins of influenza virus: 35S-methionine peptide maps as genetic markers.

Identifieur interne : 002A39 ( Main/Exploration ); précédent : 002A38; suivant : 002A40

Internal proteins of influenza virus: 35S-methionine peptide maps as genetic markers.

Auteurs : N J Dimmock ; A S Carver ; S I Kennedy ; M R Lee ; S. Luscombe

Source :

The Journal of general virology [ 0022-1317 ] ; 1977.

RBID : pubmed:915480

Descripteurs français

KwdFr :
- Cartographie chromosomique, Gènes viraux, Hémagglutinines virales (génétique), Méthionine, Nucléoprotéines (génétique), Peptides (génétique), Protéines virales (génétique), Radio-isotopes du soufre, Recombinaison génétique, Sialidase (génétique), Virion (génétique), Virulence, Virus de la grippe A (génétique).
MESH :
- génétique : Hémagglutinines virales, Nucléoprotéines, Peptides, Protéines virales, Sialidase, Virion, Virus de la grippe A.
- Cartographie chromosomique, Gènes viraux, Méthionine, Radio-isotopes du soufre, Recombinaison génétique, Virulence.

English descriptors

KwdEn :
- Chromosome Mapping, Genes, Viral, Hemagglutinins, Viral (genetics), Influenza A virus (genetics), Methionine, Neuraminidase (genetics), Nucleoproteins (genetics), Peptides (genetics), Recombination, Genetic, Sulfur Radioisotopes, Viral Proteins (genetics), Virion (genetics), Virulence.
MESH :
- chemical , genetics : Hemagglutinins, Viral, Neuraminidase, Nucleoproteins, Peptides, Viral Proteins.
- genetics : Influenza A virus, Virion.
- Chromosome Mapping, Genes, Viral, Methionine, Recombination, Genetic, Sulfur Radioisotopes, Virulence.

Abstract

Methods are described for the preparation in vivo of 35S-methionine-labelled influenza viruses, the purifiction of the nucleoprotein (NP) and matrix (M) proteins and the separation of peptides obtained by protease digestion by two-dimensional thin-layer chromatography. The maps of the M proteins of A/Okuda/57(H2N2) and A/Finland/4/74(H3N2) were very similar overall but differed in three peptides. Hence they could be clearly distinguished. Maps of the NP proteins of the same strains showed a greater number of differences. A recombinant strain having the haemagglutinin and neuraminidase of the A/Finland/4/74 parent and the virulence of the A/Okuda/57 parent was shown to have the M and P proteins of A/okuda/57.

DOI: 10.1099/0022-1317-36-3-503
PubMed: 915480

Affiliations:

Le document en format XML

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<author><name sortKey="Carver, A S" sort="Carver, A S" uniqKey="Carver A" first="A S" last="Carver">A S Carver</name>
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<author><name sortKey="Kennedy, S I" sort="Kennedy, S I" uniqKey="Kennedy S" first="S I" last="Kennedy">S I Kennedy</name>
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<author><name sortKey="Lee, M R" sort="Lee, M R" uniqKey="Lee M" first="M R" last="Lee">M R Lee</name>
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<author><name sortKey="Luscombe, S" sort="Luscombe, S" uniqKey="Luscombe S" first="S" last="Luscombe">S. Luscombe</name>
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<author><name sortKey="Carver, A S" sort="Carver, A S" uniqKey="Carver A" first="A S" last="Carver">A S Carver</name>
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<author><name sortKey="Kennedy, S I" sort="Kennedy, S I" uniqKey="Kennedy S" first="S I" last="Kennedy">S I Kennedy</name>
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<series><title level="j">The Journal of general virology</title>
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<term>Genes, Viral</term>
<term>Hemagglutinins, Viral (genetics)</term>
<term>Influenza A virus (genetics)</term>
<term>Methionine</term>
<term>Neuraminidase (genetics)</term>
<term>Nucleoproteins (genetics)</term>
<term>Peptides (genetics)</term>
<term>Recombination, Genetic</term>
<term>Sulfur Radioisotopes</term>
<term>Viral Proteins (genetics)</term>
<term>Virion (genetics)</term>
<term>Virulence</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Cartographie chromosomique</term>
<term>Gènes viraux</term>
<term>Hémagglutinines virales (génétique)</term>
<term>Méthionine</term>
<term>Nucléoprotéines (génétique)</term>
<term>Peptides (génétique)</term>
<term>Protéines virales (génétique)</term>
<term>Radio-isotopes du soufre</term>
<term>Recombinaison génétique</term>
<term>Sialidase (génétique)</term>
<term>Virion (génétique)</term>
<term>Virulence</term>
<term>Virus de la grippe A (génétique)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Hemagglutinins, Viral</term>
<term>Neuraminidase</term>
<term>Nucleoproteins</term>
<term>Peptides</term>
<term>Viral Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Influenza A virus</term>
<term>Virion</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Hémagglutinines virales</term>
<term>Nucléoprotéines</term>
<term>Peptides</term>
<term>Protéines virales</term>
<term>Sialidase</term>
<term>Virion</term>
<term>Virus de la grippe A</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Chromosome Mapping</term>
<term>Genes, Viral</term>
<term>Methionine</term>
<term>Recombination, Genetic</term>
<term>Sulfur Radioisotopes</term>
<term>Virulence</term>
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<keywords scheme="MESH" xml:lang="fr"><term>Cartographie chromosomique</term>
<term>Gènes viraux</term>
<term>Méthionine</term>
<term>Radio-isotopes du soufre</term>
<term>Recombinaison génétique</term>
<term>Virulence</term>
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<front><div type="abstract" xml:lang="en">Methods are described for the preparation in vivo of 35S-methionine-labelled influenza viruses, the purifiction of the nucleoprotein (NP) and matrix (M) proteins and the separation of peptides obtained by protease digestion by two-dimensional thin-layer chromatography. The maps of the M proteins of A/Okuda/57(H2N2) and A/Finland/4/74(H3N2) were very similar overall but differed in three peptides. Hence they could be clearly distinguished. Maps of the NP proteins of the same strains showed a greater number of differences. A recombinant strain having the haemagglutinin and neuraminidase of the A/Finland/4/74 parent and the virulence of the A/Okuda/57 parent was shown to have the M and P proteins of A/okuda/57.</div>
</front>
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<tree><noCountry><name sortKey="Carver, A S" sort="Carver, A S" uniqKey="Carver A" first="A S" last="Carver">A S Carver</name>
<name sortKey="Dimmock, N J" sort="Dimmock, N J" uniqKey="Dimmock N" first="N J" last="Dimmock">N J Dimmock</name>
<name sortKey="Kennedy, S I" sort="Kennedy, S I" uniqKey="Kennedy S" first="S I" last="Kennedy">S I Kennedy</name>
<name sortKey="Lee, M R" sort="Lee, M R" uniqKey="Lee M" first="M R" last="Lee">M R Lee</name>
<name sortKey="Luscombe, S" sort="Luscombe, S" uniqKey="Luscombe S" first="S" last="Luscombe">S. Luscombe</name>
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Serveur d'exploration H2N2

Internal proteins of influenza virus: 35S-methionine peptide maps as genetic markers.

Internal proteins of influenza virus: 35S-methionine peptide maps as genetic markers.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

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